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分离寄生虫后,对皱胃吸虫和东方杯殖吸虫进行饥饿处理,再用各种碳水化合物重新投喂,之后它们的糖原含量情况。

Glycogen content in Gastrothylax crumenifer and Cotylophoron orientale after the isolation of parasites, their starvation and refeeding with various carbohydrates.

作者信息

Bahadur R, Gupta P C

出版信息

Folia Parasitol (Praha). 1984;31(3):259-63.

PMID:6479754
Abstract

The average reserve glycogen content in unstarved (control) parasites, 4.7 +/- 0.14 g/100 g F.T. (fresh weight of tissue) in Gastrothylax crumenifer and 3.15 +/- 0.14 g/100 g F.T. in Cotylophoron orientale has been estimated biochemically. The consumption of endogenous glycogen was found to be 18.47%, 44.37%, 66.66% and 78.13% of F. T. in G. crumenifer and 14.92%, 40.95%, 66.34% and 71.42% of F.T. in C. orientale during 12, 24, 36 and 48 hours of starvation. Both the parasites were able to resynthesize 71.71% and 75.42% of lost glycogen (maximum up to 16 hours) on refeeding in nutrient medium containing glucose. The maximum resynthesis of lost glycogen in both the parasites was observed in nutrient medium containing glucose rather than the other carbohydrates such as fructose, lactose, sucrose and trehalose.

摘要

已通过生化方法估算出未饥饿(对照)寄生虫体内的糖原储备平均含量,在皱胃吸虫中为4.7±0.14克/100克鲜组织重,在东方杯殖吸虫中为3.15±0.14克/100克鲜组织重。结果发现,在饥饿12、24、36和48小时期间,皱胃吸虫内源性糖原的消耗量分别为鲜组织重的18.47%、44.37%、66.66%和78.13%,东方杯殖吸虫内源性糖原的消耗量分别为鲜组织重的14.92%、40.95%、66.34%和71.42%。在含有葡萄糖的营养培养基中重新喂食后,这两种寄生虫都能够重新合成71.71%和75.42%(最长可达16小时)的损失糖原。在含有葡萄糖的营养培养基中观察到这两种寄生虫损失糖原的重新合成量最大,而不是在含有果糖、乳糖、蔗糖和海藻糖等其他碳水化合物的培养基中。

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