Wenstrom J C, Hamilton D W
Int J Androl. 1984 Jun;7(3):215-35. doi: 10.1111/j.1365-2605.1984.tb00779.x.
Epithelial strips of rat vas deferens were isolated by a new technique and used to study differences in protein synthesis and secretion between morphologically defined segments of the vas deferens. The isolated strips were viable as judged by linear oxygen uptake over the incubation period and by preservation of structure. Epithelium from the proximal vas deferens incorporated more labelled amino acids into cytosolic (P less than 0.02) and incubation medium (P less than 0.01) proteins than did epithelium from distal vas deferens; this incorporation was inhibited by cycloheximide. Although some of the incubation medium proteins arose by leakage from damaged cells, specific protein secretion was indicated by differences in SDS-PAGE autoradiogram banding patterns and by differences in glycosylation between proteins in the incubation medium and those in the cytosol. Thus, the former contained more label from [14C]galactose incorporation than did cytosolic proteins (proximal: P less than 0.05; distal: P less than 0.005).
采用一种新技术分离大鼠输精管的上皮条带，用于研究输精管形态学上不同节段之间蛋白质合成与分泌的差异。通过孵育期间线性耗氧量以及结构保存情况判断，分离出的条带具有活性。近端输精管的上皮比远端输精管的上皮将更多标记氨基酸掺入胞质蛋白（P<0.02）和孵育培养基蛋白（P<0.01）中；这种掺入被环己酰亚胺抑制。虽然一些孵育培养基蛋白是由受损细胞泄漏产生的，但SDS-PAGE放射自显影片带型的差异以及孵育培养基中蛋白质与胞质中蛋白质糖基化的差异表明存在特异性蛋白质分泌。因此，前者从[14C]半乳糖掺入中获得的标记比胞质蛋白更多（近端：P<0.05；远端：P<0.005）。
