Meijer A E, Elias E A
Histochem J. 1984 Sep;16(9):971-82. doi: 10.1007/BF01003852.
The myotoxic effect of the subcutaneous administration of N,N1-dimethyl-p-phenylenediamine (DPPD) in rats was enhanced by the simultaneous administration of hyaluronidase. The resulting myopathy was associated with an early and dramatic increase in activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Administration of actinomycin D or cycloheximide prior to the combined DPPD and hyaluronidase treatment prevented the increase in activity of both pentose phosphate pathway enzymes, indicating that the increase in activity requires RNA synthesis and protein synthesis. The possibility that the increase in activity of both NADPH-regenerating enzymes results from the modification by effectors of existing less active forms of these enzymes leading to more highly active forms was refuted.
皮下注射N，N1-二甲基对苯二胺（DPPD）对大鼠产生的肌毒性作用，在同时给予透明质酸酶的情况下会增强。由此产生的肌病与葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶活性的早期显著增加有关。在联合给予DPPD和透明质酸酶治疗之前给予放线菌素D或环己酰亚胺，可防止磷酸戊糖途径两种酶的活性增加，这表明活性增加需要RNA合成和蛋白质合成。烟酰胺腺嘌呤二核苷酸磷酸（NADPH）再生酶活性增加是由这些酶现有的活性较低形式的效应物修饰导致更具活性形式的可能性被否定了。
