细胞和组织中谷胱甘肽及其他硫醇的测定：基于硫醇单溴代联苯胺衍生物高效液相色谱分离的简化方法。

Measurements of glutathione and other thiols in cells and tissues: a simplified procedure based on the HPLC separation of monobromobimane derivatives of thiols.

作者信息

Minchinton A I

出版信息

Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1503-6. doi: 10.1016/0360-3016(84)90490-5.

DOI:10.1016/0360-3016(84)90490-5

Abstract

Although there is intense interest in the role of thiols in controlling the efficiency of radiosensitizers, and in developing thiols (or pro-drugs liberating thiols) as radioprotectors, there is little information regarding the concentration of specific thiols in cells, tumors and normal tissues. Details are presented of a modified procedure using the thiol binding agent monobromobimane with separation using paired-ion reverse phase high performance liquid chromatography (HPLC). This method has been extended to include measurements of the radiosensitizer misonidazole and its desmethylated metabolite Ro 05-9963 in tissues.

摘要

尽管人们对硫醇在控制放射增敏剂效率方面的作用以及开发硫醇（或释放硫醇的前药）作为辐射防护剂有着浓厚兴趣，但关于细胞、肿瘤和正常组织中特定硫醇浓度的信息却很少。本文详细介绍了一种改良方法，该方法使用硫醇结合剂单溴双马来酰亚胺，并通过离子对反相高效液相色谱（HPLC）进行分离。此方法已扩展到可测量组织中的放射增敏剂米索硝唑及其去甲基代谢物Ro 05 - 9963。

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Spontaneous overexpression of the long form of the Bcl-X protein in a highly resistant P388 leukaemia.在一种高度耐药的P388白血病中，Bcl-X蛋白长形式的自发过表达。

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