使用一步制备凝胶电泳法分离蛋白质uH2A。
Isolation of protein uH2A using a one step preparative gel electrophoresis.
作者信息
Dalay N, Ozkaynak E, Kirdar B, Carin M, Bermek E
出版信息
Prep Biochem. 1984;14(2):181-92. doi: 10.1080/10826068408070624.
PMID:6483804
Abstract
A one step electrophoretic procedure for the isolation of protein uH2A has been devised which may improve the overall yield. The improvement involves elimination of intermediate steps which might result in the decrease of the yield. The method may serve as an alternate to the conventional methods and can also be used successfully for the isolation of several different proteins.
摘要
已设计出一种用于分离蛋白质uH2A的一步电泳程序，这可能会提高总产量。这种改进包括消除可能导致产量下降的中间步骤。该方法可作为传统方法的替代方法，也可成功用于分离几种不同的蛋白质。
相似文献
1
Isolation of protein uH2A using a one step preparative gel electrophoresis.使用一步制备凝胶电泳法分离蛋白质uH2A。
Prep Biochem. 1984;14(2):181-92. doi: 10.1080/10826068408070624.
2
An electrophoretic comparison of non-histone proteins from rat liver total chromatin and chromatin depleted of 0.35 M NaCl soluble proteins.大鼠肝脏总染色质与去除0.35M NaCl可溶性蛋白的染色质中非组蛋白的电泳比较。
Int J Biochem. 1981;13(8):941-6. doi: 10.1016/0020-711x(81)90022-7.
3
Separation of nucleic acids and chromatin proteins by hydrophobic interaction chromatography.通过疏水相互作用色谱法分离核酸和染色质蛋白。
Biochim Biophys Acta. 1982 Mar 29;696(3):323-31. doi: 10.1016/0167-4781(82)90064-1.
4
Failure of preparative flat bed electrofocusing to resolve rat liver chromosomal proteins.制备性平板床电聚焦未能分离大鼠肝脏染色体蛋白。
Anal Biochem. 1978 Jun 15;87(1):157-61. doi: 10.1016/0003-2697(78)90580-8.
5
Thermostability of chromatin and variations in chromosomal proteins isolated under different ionic conditions.染色质的热稳定性以及在不同离子条件下分离得到的染色体蛋白质的变化。
J Biochem. 1980 Nov;88(5):1283-9. doi: 10.1093/oxfordjournals.jbchem.a133097.
6
A new high sensitive analytical micro-scale procedure for chromosomal proteins.一种用于染色体蛋白的新型高灵敏度分析微观方法。
Mol Biol Rep. 1978 Oct 16;4(3):171-5. doi: 10.1007/BF00777519.
7
A simplified SDS-gel electrophoresis procedure with improved resolution of chromosomal proteins.一种分辨率得到提高的简化的染色体蛋白质十二烷基硫酸钠凝胶电泳程序。
Int J Biochem. 1978;9(11):833-7. doi: 10.1016/0020-711x(78)90033-2.
8
Purification of a phosphoprotein from chromatin of rat liver.从大鼠肝脏染色质中纯化一种磷蛋白。
Biochem J. 1979 Oct 1;183(1):147-56. doi: 10.1042/bj1830147.
9
A method for the fractionation of the high-mobility-group non-histome chromosomal proteins.一种用于分离高迁移率族非组蛋白染色体蛋白的方法。
Biochem Biophys Res Commun. 1977 Oct 10;78(3):1034-42. doi: 10.1016/0006-291x(77)90525-3.
10
Two-dimensional gel electrophoresis of nonhistone chromatin proteins with nonequilibrium pH gradient electrophoresis as the first dimension.以非平衡pH梯度电泳作为第一向的非组蛋白染色质蛋白质的二维凝胶电泳。
Anal Biochem. 1982 Jan 15;119(2):433-9. doi: 10.1016/0003-2697(82)90610-8.
引用本文的文献
1
Binding of protein uH2A and histone H2A to DNA.蛋白质uH2A和组蛋白H2A与DNA的结合。
Cell Biophys. 1989 Feb;14(1):43-51. doi: 10.1007/BF02797390.
Zotero 插件