Mulroy M J, Whaley E A
Scan Electron Microsc. 1984(Pt 2):831-40.
The stereocilia of auditory hair cells were examined with a scanning electron microscope during periods of noise-induced temporary threshold shift. The stereocilia in the tallest row of each tuft of the noise-exposed ears were clumped together compared with control ears. This suggests to us that clumping of the longest stereocilia might be a reversible mechanism of the hair cell to protect itself from overstimulation. The rate of recovery following exposure to noise was monitored by measuring the first neural component of the cochlear potential response. The duration of the noise was adjusted to ensure sufficient time to fix the ears during the period of temporary deafness. Alcian blue was added to the fixative to preserve the glycocalyx of the stereocilia. The experiments were done in the basal region of the auditory organ of the alligator lizard. This region is characterized by hair cells with long free-standing stereocilia.
在噪声诱发的暂时性阈移期间，用扫描电子显微镜检查听觉毛细胞的静纤毛。与对照耳相比，噪声暴露耳每束静纤毛中最高一排的静纤毛聚集在一起。这向我们表明，最长静纤毛的聚集可能是毛细胞保护自身免受过度刺激的一种可逆机制。通过测量耳蜗电位反应的第一个神经成分来监测噪声暴露后的恢复率。调整噪声持续时间，以确保在暂时性耳聋期间有足够时间固定耳朵。将阿尔辛蓝添加到固定剂中以保存静纤毛的糖萼。实验在扬子鳄听觉器官的基部区域进行。该区域的特征是具有长的独立静纤毛的毛细胞。
