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葡萄糖和温度对仓鼠成纤维细胞中杂合基因的调控

Regulation of a hybrid gene by glucose and temperature in hamster fibroblasts.

作者信息

Attenello J W, Lee A S

出版信息

Science. 1984 Oct 12;226(4671):187-90. doi: 10.1126/science.6484570.

DOI:10.1126/science.6484570
PMID:6484570
Abstract

A novel eukaryotic hybrid gene has been constructed from the 5' sequence of a rat gene and the bacterial neomycin-resistance gene. After transfection into hamster fibroblasts, the neo transcripts can be induced to high levels by the absence of glucose. Furthermore, this hybrid gene can be regulated by temperature when it is introduced into a temperature-sensitive mutant cell line.

摘要

一种新型真核杂交基因由大鼠基因的5'序列和细菌新霉素抗性基因构建而成。转染到仓鼠成纤维细胞后，在缺乏葡萄糖的情况下，新霉素转录本可被诱导至高水平。此外，当将该杂交基因导入温度敏感突变细胞系时，它可受温度调控。

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Regulation of a hybrid gene by glucose and temperature in hamster fibroblasts.葡萄糖和温度对仓鼠成纤维细胞中杂合基因的调控
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