Miranda C L, Henderson M C, Buhler D R
Toxicol Appl Pharmacol. 1984 Oct;76(1):62-8. doi: 10.1016/0041-008x(84)90029-2.
Young male rats were pair-fed diets containing 0 or 5% tansy ragwort (TR), with each diet containing 0 or 50 micrograms/g copper. Other animals given TR-free diets were also maintained as ad libitum controls. After 5 weeks of feeding these diets, the rats were killed for the determination of the trace metal contents of liver fractions. TR with no dietary copper supplement increased the calcium content of all hepatic fractions except nuclei and debris, but had no effect on the subcellular concentrations of copper, zinc, or iron. When 50 micrograms/g copper was included in the TR diet, the subcellular distribution of copper, iron, and calcium was altered. This alteration was shown by an increase (as compared to pair-fed controls given copper-supplemented diet with no TR) in the copper content of all fractions except cytosol, the iron content of all fractions, and the calcium content of mitochondria, microsomes, lysosomes, and cytosol. Feed restriction (pair-fed versus ad libitum controls) using diets with no additional copper produced an increase in the copper and calcium contents of mitochondria; the copper, zinc, and calcium contents of cytosol; and the calcium content of lysosomes. Addition of 50 micrograms/g copper to the diets abolished these effects of feed restriction. These findings suggest that the subcellular distribution of copper, zinc, iron, and calcium in rat liver is subject to perturbation by pyrrolizidine alkaloid-containing diet, dietary copper, and feed restriction.
将年轻雄性大鼠成对喂养含0%或5%千里光（TR）的日粮，每种日粮含0或50微克/克铜。给其他食用不含TR日粮的动物也作为自由采食对照。喂养这些日粮5周后，处死大鼠以测定肝组分中的痕量金属含量。不补充日粮铜的TR增加了除细胞核和碎片外所有肝组分的钙含量，但对铜、锌或铁的亚细胞浓度没有影响。当TR日粮中包含50微克/克铜时，铜、铁和钙的亚细胞分布发生改变。与成对喂养补充铜但不含TR的日粮的对照相比，这种改变表现为除胞质溶胶外所有组分的铜含量、所有组分的铁含量以及线粒体、微粒体、溶酶体和胞质溶胶的钙含量增加。使用不添加额外铜的日粮进行限食（成对喂养与自由采食对照）导致线粒体的铜和钙含量、胞质溶胶的铜、锌和钙含量以及溶酶体的钙含量增加。日粮中添加50微克/克铜消除了限食的这些影响。这些发现表明，大鼠肝脏中铜、锌、铁和钙的亚细胞分布会受到含吡咯里西啶生物碱的日粮、日粮铜和限食的干扰。
