Hassoun E, d'Argy R, Dencker L, Lundin L G, Borwell P
Toxicol Lett. 1984 Oct;23(1):37-42. doi: 10.1016/0378-4274(84)90007-9.
A series of recombinant inbred strains called BXD [produced from a cross between C57BL/6J (B6) and DBA/2J (D2)] were given single i.p. doses of 0.6 mg/kg 2,3,7, 8-tetrachlorodibenzofuran (TCDBF) on day 12 of gestation. The uteri were examined in late gestation with respect to resorptions and fetal death, and fetal malformations. The strains of the B6-type with respect to Ah-locus (Nos. 5, 6, 8, 11, 12, 14, 16 and 29) that are Ah-responsive, exhibited cleft palates in 80-100% of all fetuses, while hydronephrosis occurred at a rate of 20-70%. These two types of malformation are well recognized from earlier experiments with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and its structural analogues, including TCDBF. In the strains of D2-type with respect to Ah-locus (Nos. 2, 15, 19, 21, 22, 24, and 31), which are Ah-nonresponsive, no cleft palates occurred. One strain (No. 2) had a few (17%) fetuses with hydronephrosis. The frequency of fetal deaths and resorptions were relatively low, but slightly higher among B6-strains than D2-strains. The results indicate an association between the genes producing malformations by TCDBF and the Ah-locus.
一系列名为BXD的重组近交系（由C57BL/6J（B6）和DBA/2J（D2）杂交产生）在妊娠第12天经腹腔注射单剂量0.6mg/kg的2,3,7,8-四氯二苯并呋喃（TCDBF）。在妊娠后期检查子宫，观察吸收情况、胎儿死亡情况以及胎儿畸形情况。关于Ah位点的B6型菌株（第5、6、8、11、12、14、16和29号）对Ah有反应，在所有胎儿中有80%-100%出现腭裂，而肾盂积水的发生率为20%-70%。从早期使用2,3,7,8-四氯二苯并对二恶英（TCDD）及其结构类似物（包括TCDBF）的实验中，这两种畸形是广为人知的。关于Ah位点的D2型菌株（第2、15、19、21、22、24和31号）对Ah无反应，未出现腭裂。一个菌株（第2号）有少数（17%）胎儿出现肾盂积水。胎儿死亡和吸收的频率相对较低，但B6型菌株中的频率略高于D2型菌株。结果表明，TCDBF产生畸形的基因与Ah位点之间存在关联。
