[Isolated acinus cells in the significance of ischemia in the pathogenesis of acute pancreatitis].

Additionally to the pancreatic edema short-time ischemia represents an essential factor in formation of an experimentally induced acute pancreatitis. The consequences of such an ischemia at the guinea pig pancreas on the cellular energy metabolism were investigated in isolated exocrine cells. It was shown that intact acinus cells can be isolated as well as from normally perfused and ischemic pretreated pancreata. The energy metabolism was characterized by the difference between resting and maximum respiration after stepwise uncoupling. This parameter represents a dynamic measure and allows an estimate of the load capacity of the cellular energy metabolism. Exocrine cells isolated after ischemia (20 minutes), had a smaller load capacity than cells from normally perfused pancreata. This indicates an alteration of energy supply after ischemia. The consequences of this reduced supply on energy dependent repairing and protective mechanisms are discussed in terms of the pathogenesis of acute pancreatitis.