Elkon K B, Jankowski P W, Chu J L
Anal Biochem. 1984 Jul;140(1):208-13. doi: 10.1016/0003-2697(84)90156-8.
A composite agarose-polyacrylamide gel containing urea and sodium dodecyl sulfate reliably resolved unreduced human immunoglobulins according to their molecular weight. Intact immunoglobulins and a number of other macromolecules were readily transferred to nitrocellulose paper by either capillary or electrophoretic blotting, although the latter technique was more effective. Conventional antigen probing as well as immobilized antibody studies can be performed on the nitrocellulose transfers.
一种含有尿素和十二烷基硫酸钠的复合琼脂糖 - 聚丙烯酰胺凝胶能够根据未还原的人类免疫球蛋白的分子量可靠地将其分离。完整的免疫球蛋白和许多其他大分子通过毛细管印迹或电泳印迹都能很容易地转移到硝酸纤维素纸上，不过后一种技术更有效。可以在硝酸纤维素转移膜上进行传统的抗原探测以及固定化抗体研究。
