DNA片段的链分离及其从非变性聚丙烯酰胺凝胶中的分离。
Strand separation of DNA fragments and their isolation from nondenaturing polyacrylamide gels.
作者信息
James R, Bradshaw R A
出版信息
Anal Biochem. 1984 Aug 1;140(2):456-8. doi: 10.1016/0003-2697(84)90193-3.
PMID:6486433
Abstract
A technique for rapidly and quantitatively denaturing double-stranded DNA employing urea and moderate heat is described. The single DNA strands are resolved on high-percentage nondenaturing polyacrylamide gels from which they can be recovered for Maxam-Gilbert sequence analysis.
摘要
本文描述了一种利用尿素和适度加热快速定量变性双链DNA的技术。单链DNA在高百分比非变性聚丙烯酰胺凝胶上分离，可从中回收用于Maxam-Gilbert序列分析。
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