Sukhareva-Nemakova N N, Chuenkova M V
Antibiotiki. 1984 Jul;29(7):501-6.
The paper deals with possible discovery of ways for increasing sensitivity of trypanosomides to polyenic antibiotics. The following substances were tested: sodium pyruvate and acetate, calcium salts, ascorbic acid and 1-valine. The total number of the cells and the number of the viable cells in the culture and their morphological characteristics were used as the criteria for estimation of the C. oncopelti sensitivity. It was shown that sodium acetate most actively modified the levorin effect on C. oncopelti. Its addition in a concentration of 40 mg/ml to the cultivation medium with levorin in a concentration of 1 microgram/ml induced a trypanocidal effect. With the use of levorin alone such an effect was observed when the antibiotic was used in a concentration of 10 micrograms/ml. The growth rate of the protozoon was decreased by 60-80 per cent as compared to the control. The number of the viable cells was lowered 4 times. The morphology of the culture markedly changed. This indicates that the presence of sodium acetate as a modifier in the culture medium allowed one to decrease 10 times the dose of levorin and to preserve the trypanocidal effect.
本文探讨了提高锥虫对多烯抗生素敏感性的可能途径。测试了以下物质：丙酮酸钠和乙酸钠、钙盐、抗坏血酸和L-缬氨酸。培养物中的细胞总数、活细胞数及其形态特征被用作评估康氏锥虫敏感性的标准。结果表明，乙酸钠最显著地改变了左霉素对康氏锥虫的作用。在含有1微克/毫升左霉素的培养基中加入浓度为40毫克/毫升的乙酸钠可诱导杀锥虫作用。单独使用左霉素时，当抗生素浓度为10微克/毫升时才观察到这种效果。与对照相比，原生动物的生长速率降低了60%-80%。活细胞数量减少了4倍。培养物的形态发生了显著变化。这表明在培养基中加入乙酸钠作为调节剂可使左霉素剂量降低10倍并保持杀锥虫作用。
