Romero F J, Sies H
Biochem Biophys Res Commun. 1984 Sep 28;123(3):1116-21. doi: 10.1016/s0006-291x(84)80248-x.
The glutathione contents of the mitochondrial and cytosolic fractions and extracellular space of isolated hepatocytes decrease when glutathione synthesis is inhibited with L-buthionine sulfoximine. Mitochondrial glutathione is depleted to 50% of its initial value whereas the cytosolic pool is completely emptied after 2 h incubation in the presence of inhibitor. The mitochondrial glutathione content was only fully depleted when L-buthionine sulfoximine was added together with phorone (2,6-dimethyl-2,5-heptadiene-4-one), a substrate of the glutathione S-transferases (E.C. 2.5.1.18).
当用L-丁硫氨酸亚砜亚胺抑制谷胱甘肽合成时，分离的肝细胞线粒体、胞质部分及细胞外空间中的谷胱甘肽含量会降低。线粒体谷胱甘肽耗竭至其初始值的50%，而在抑制剂存在下孵育2小时后，胞质库完全排空。只有当L-丁硫氨酸亚砜亚胺与谷胱甘肽S-转移酶（E.C. 2.5.1.18）的底物佛尔酮（2,6-二甲基-2,5-庚二烯-4-酮）一起添加时，线粒体谷胱甘肽含量才会完全耗竭。
