Kishimoto R, Murano S, Nagai K, Nakagawa H
Biochem Int. 1984 Aug;9(2):153-60.
Extraction of the cytoplasm of rat liver with 5% PCA and subsequent TCA (20%) treatment resulted in the recovery of only a few proteins, the predominant one having an apparent molecular weight of 12K. This 12K protein increased sharply (9-fold) between 2 weeks and 10-20 weeks and then decreased until at least 2 years after birth. 12K protein was separated into two components, 12K-1 (larger) and 12K-2 (smaller), by gel chromatography which had similar amino acid compositions to those of profilin (from Acanthamoeba and hog thyroid). Like profilin, 12K-1 protein inhibited the rate of actin polymerization, while 12K-2 protein did not. These findings suggest that liver profilin-like proteins increase sharply during development.
用5%的PCA提取大鼠肝脏的细胞质，随后用20%的TCA处理，结果只回收了少数几种蛋白质，其中主要的一种蛋白质的表观分子量为12K。这种12K蛋白质在2周龄至10 - 20周龄之间急剧增加（9倍），然后下降，直至出生后至少2年。通过凝胶色谱法，12K蛋白质被分离成两个组分，12K - 1（较大）和12K - 2（较小），它们的氨基酸组成与肌动蛋白结合蛋白（来自棘阿米巴和猪甲状腺）的氨基酸组成相似。与肌动蛋白结合蛋白一样，12K - 1蛋白质抑制肌动蛋白聚合速率，而12K - 2蛋白质则不抑制。这些发现表明，肝脏中类似肌动蛋白结合蛋白的蛋白质在发育过程中急剧增加。
