实验性原发性阿米巴脑膜脑炎期间小鼠脑髓磷脂的生化变化。
Biochemical changes in mouse brain myelin during experimental primary amoebic meningo-encephalitis.
作者信息
Tekwani B L, Tripathi L M, Mohan Rao V K
出版信息
Br J Exp Pathol. 1984 Oct;65(5):637-44.
Myelin membranes were purified from normal as well as experimental primary amoebic meningo-encephalitis-afflicted mouse brains. Infected myelin fractions were observed to form a floating fraction in 0.32 M sucrose. Total proteins, total lipids, phospholipids, cholesterol and galactolipids decreased markedly due to amoebic infection. Phosphatidyl choline, phosphatidyl ethanolamine plus phosphatidyl serine and sphingomyelin were decreased with a concomitant increase in lysophosphatidyl choline, lysophosphatidyl ethanolamine plus lysophosphatidyl serine and phosphatidic acid. SDS-polyacrylamide gel electrophoresis of myelin proteins exhibited six major protein fractions. Marked depletion in proteolipid protein was observed; 5'-nucleotidase activity increased while Na,K-ATPase and Mg-ATPase activities decreased. Infected myelin Na,K-ATPase had lower Vmax and higher Km values than normal. Arrhenius plots of normal myelin Na,K-ATPase exhibited transition temperature at 22 degrees C while no transition temperature could be observed in case of infected myelin Na,K-ATPase.
从正常以及患有实验性原发性阿米巴脑膜脑炎的小鼠大脑中纯化髓磷脂膜。观察到受感染的髓磷脂部分在0.32M蔗糖中形成漂浮部分。由于阿米巴感染，总蛋白、总脂质、磷脂、胆固醇和半乳糖脂显著减少。卵磷脂、磷脂酰乙醇胺加磷脂酰丝氨酸和鞘磷脂减少，同时溶血卵磷脂、溶血磷脂酰乙醇胺加溶血磷脂酰丝氨酸和磷脂酸增加。髓磷脂蛋白的SDS-聚丙烯酰胺凝胶电泳显示出六个主要蛋白部分。观察到蛋白脂蛋白明显减少；5'-核苷酸酶活性增加，而钠钾-ATP酶和镁-ATP酶活性降低。受感染的髓磷脂钠钾-ATP酶的Vmax较低，Km值较高。正常髓磷脂钠钾-ATP酶的阿累尼乌斯图显示转变温度为22℃，而受感染的髓磷脂钠钾-ATP酶则未观察到转变温度。
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