Carter R D, Brooks J R, Dill K
Biochim Biophys Acta. 1984 Nov 9;790(3):285-7. doi: 10.1016/0167-4838(84)90033-5.
Glycophorin BN was reductively [13C]methylated and the 13C chemical shift of the N-terminal [13C]dimethyl-leucine residue was monitored as a function of pH. These results were compared to the pH-dependent chemical shift studies of the N-terminal [13C]dimethylleucine residues of intact glycophorin AN and N-terminal glyco-octapeptide AN. The results indicate that the titration data for [13C]dimethylleucine of glycophorin BN more closely resembles the titration data observed for the [13C]dimethylleucine residue of the N-terminal glyco-octapeptide AN rather than for the [13C]dimethylleucine residue of intact glycophorin AN. Integration of the 13C resonances indicated that glycophorin BN contains 3-4 lysine residues.
血型糖蛋白BN经还原[13C]甲基化处理，并监测N端[13C]二甲基亮氨酸残基的13C化学位移随pH的变化。将这些结果与完整血型糖蛋白AN的N端[13C]二甲基亮氨酸残基以及N端糖基八肽AN的pH依赖性化学位移研究结果进行比较。结果表明，血型糖蛋白BN的[13C]二甲基亮氨酸的滴定数据更类似于N端糖基八肽AN的[13C]二甲基亮氨酸残基的滴定数据，而不是完整血型糖蛋白AN的[13C]二甲基亮氨酸残基的滴定数据。13C共振积分表明血型糖蛋白BN含有3 - 4个赖氨酸残基。
