Hennink W E, Ebert C D, Kim S W, Breemhaar W, Bantjes A, Feijen J
Biomaterials. 1984 Sep;5(5):264-8. doi: 10.1016/0142-9612(84)90070-x.
The adsorption of antithrombin III (AT III) onto polystyrene surfaces preadsorbed with albumin or albumin-heparin conjugates was studied using a two step enzyme immuno assay. When AT III-buffer solutions were used, the highest adsorption values were measured on high affinity albumin-heparin conjugate pretreated surfaces. Less AT III adsorption was found on nonfractionated albumin-heparin conjugate preadsorbed surfaces. AT III adsorption could also be detected on low affinity conjugate and albumin coated surfaces. When AT III was adsorbed from plasma or plasma dilutions with buffer, only AT III on surfaces preadsorbed with high affinity or nonfractionated albumin-heparin conjugate was found. These results demonstrate that the heparin moiety of the conjugate is directed to the solution phase whereas the albumin moiety contacts the polystyrene surface.
采用两步酶免疫分析法研究了抗凝血酶III（AT III）在预先吸附有白蛋白或白蛋白-肝素缀合物的聚苯乙烯表面上的吸附情况。当使用AT III缓冲溶液时，在高亲和力白蛋白-肝素缀合物预处理的表面上测得的吸附值最高。在未分级的白蛋白-肝素缀合物预吸附表面上发现的AT III吸附较少。在低亲和力缀合物和白蛋白包被的表面上也能检测到AT III吸附。当从血浆或用缓冲液稀释的血浆中吸附AT III时，仅在预先吸附有高亲和力或未分级的白蛋白-肝素缀合物的表面上发现了AT III。这些结果表明，缀合物中的肝素部分指向溶液相，而白蛋白部分与聚苯乙烯表面接触。
