加拉明、加洛帕米和硝苯地平对豚鼠盲肠带对乙酰胆碱和卡巴胆碱反应的影响。
The effect of gallamine, gallopamil and nifedipine on responses to acetylcholine and carbachol in the taenia of the guinea-pig caecum.
作者信息
Mitchelson F, Ziegler A
出版信息
Br J Pharmacol. 1984 Sep;83(1):145-55. doi: 10.1111/j.1476-5381.1984.tb10129.x.
The effects of gallamine, gallopamil and nifedipine on isotonic contractions of the isolated taenia of the guinea-pig caecum produced by acetylcholine (ACh) or carbachol (CCh) were investigated. Gallamine (0.1 to 0.3 mM) inhibited contractions produced by CCh more than those produced by ACh. The difference was still present after pretreatment of the tissue with paraoxon (10 microM for 20 min) to inhibit cholinesterases or in experiments carried out in the presence of tetrodotoxin (0.3 microM) to exclude possible ganglionic stimulation by the agonists. Gallopamil or nifedipine selectively inhibited the tonic response to ACh in the absence or presence of paraoxon. The phasic response to ACh or the tonic response to CCh (0.1 or 1 microM) was much less affected. Reduction of the Ca2+ content of the bath medium reduced phasic and tonic responses to ACh more than the tonic response to CCh. These results suggest that there are differences in the interaction of ACh and CCh with muscarinic receptors in this muscle.
研究了加拉明、加洛帕米和硝苯地平对乙酰胆碱（ACh）或卡巴胆碱（CCh）引起的豚鼠盲肠离体绦虫等张收缩的影响。加拉明（0.1至0.3 mM）对CCh引起的收缩的抑制作用比对ACh引起的收缩的抑制作用更强。在用对氧磷（10 microM，作用20分钟）预处理组织以抑制胆碱酯酶后，或在存在河豚毒素（0.3 microM）以排除激动剂可能的神经节刺激的实验中，这种差异仍然存在。在不存在或存在对氧磷的情况下，加洛帕米或硝苯地平选择性地抑制对ACh的强直反应。对ACh的相性反应或对CCh（0.1或1 microM）的强直反应受影响较小。浴液介质中Ca2+含量的降低对ACh的相性和强直反应的降低作用比对CCh的强直反应的降低作用更大。这些结果表明，在该肌肉中，ACh和CCh与毒蕈碱受体的相互作用存在差异。
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