Comparison of three methods for investigating placental protein 12(PP12)-like immunoreactive substance from preovulatory follicular fluid and purified PP12 from human placenta.

Placental protein 12(PP12)-like immunoreactive fraction of preovulatory follicular fluid from Sephadex G-100 and purified placental PP12 were subjected to reverse phase high performance liquid chromatography (HPLC). The elution was carried out with acetonitrile gradient. PP12-like immunoreactivity of both samples had the same retention time. This fraction of follicular fluid from HPLC and placental PP12 were then run on a sodium dodecyl sulfate-polyacrylamide gel and transferred electrophoretically to a nitrocellulose (NC) sheet. Protein bands on the NC sheet were then detected immunologically with anti-PP12 antisera. After visualising the bands which had reacted with antisera it was obvious that PP12-like immunoreactive substance from follicular fluid and from placenta had an electrophoretic mobility corresponding to the same molecular mass.