Crouch R K, Patrick J, Goosey J, Coles W H
Curr Eye Res. 1984 Sep;3(9):1119-23. doi: 10.3109/02713688409000811.
The enzymatic activity of copper-zinc superoxide dismutase within the cornea and lens of the juvenile (1 week) and adult (26 week) rat has been measured relative to both total soluble protein and immunoreactive protein. Enzymatic activity of corneal superoxide dismutase, localized in the epithelium and endothelium, remains constant with age relative to total soluble protein and the ratio of catalytically active enzyme to immunoreactive enzyme is near unity indicating that the superoxide dismutase present is enzymatically active. Likewise, in the juvenile rat, the majority of lens superoxide dismutase, which is located in the capsule epithelium, is enzymatically active. However, in the adult rat a large portion of the enzyme present is catalytically inactive. The enzymatic activity also decreases relative to total soluble protein with age in both the rat and the rabbit lens. This decrease in protection against toxic radicals and/or the accumulation of enzymatically defective enzyme may be contributing factors to age related lens disorders.
已针对总可溶性蛋白和免疫反应性蛋白测定了幼年（1周龄）和成年（26周龄）大鼠角膜和晶状体中铜锌超氧化物歧化酶的酶活性。角膜超氧化物歧化酶的酶活性定位于上皮细胞和内皮细胞，相对于总可溶性蛋白而言，其活性随年龄增长保持恒定，且催化活性酶与免疫反应性酶的比例接近1，这表明所存在的超氧化物歧化酶具有酶活性。同样，在幼年大鼠中，位于晶状体囊上皮的大部分晶状体超氧化物歧化酶具有酶活性。然而，在成年大鼠中，所存在的大部分酶没有催化活性。大鼠和兔晶状体中的酶活性也随年龄增长相对于总可溶性蛋白而降低。这种对有毒自由基保护作用的降低和/或酶缺陷型酶的积累可能是与年龄相关的晶状体疾病的促成因素。
