Meier S, Drake C
Dev Biol. 1984 Nov;106(1):83-8. doi: 10.1016/0012-1606(84)90064-2.
Embryonic chick corneal explants were soaked in mild detergent and the anterior corneal epithelium was peeled from its basement membrane, leaving the lamina lucida surface exposed and supported on the subjacent primary stroma. Explants were treated with rabbit anti-laminin IgG, followed by sheep anti-rabbit IgG linked microspheres, and processed for SEM. The lucida surface was heavily decorated with microspheres, whereas controls treated with preimmune rabbit IgG were essentially beadless. Laminin distribution was not regular, appearing denser in some regions than others. However, the connective tissue surface of the basement membrane was never laminin-positive, even after treatment with hyaluronidase. These results suggest the basal lamina of the corneal epithelium is asymmetric, with preferential location of laminin to the lucida surface of the basement membrane.
将鸡胚角膜外植体浸泡在温和的去污剂中，然后将角膜前上皮从其基底膜上剥离，使透明板表面暴露，并由下方的初级基质支撑。外植体先用兔抗层粘连蛋白IgG处理，再用与羊抗兔IgG相连的微球处理，然后进行扫描电子显微镜（SEM）处理。透明板表面布满了微球，而用免疫前兔IgG处理的对照基本上没有珠子。层粘连蛋白的分布不规则，在某些区域比其他区域更密集。然而，即使在用透明质酸酶处理后，基底膜的结缔组织表面也从未呈层粘连蛋白阳性。这些结果表明角膜上皮的基底层是不对称的，层粘连蛋白优先位于基底膜的透明板表面。
