Nielsen N C, Risum J, Bogaard Hansen K, Nissen U
Gynecol Obstet Invest. 1984;18(3):147-51. doi: 10.1159/000299070.
The results obtained by insemination with 3,418 frozen donor ejaculates with known semen characteristics were analyzed. The concentrations of motile spermatozoa before and after thawing were significantly higher in samples from donors producing pregnancies compared to samples from donors not causing pregnancies. In ejaculates known specifically to have caused a pregnancy even higher concentrations were found. It was assumed that the concentration of motile spermatozoa after thawing was the most important parameter, and by dividing the material into fractions it could be demonstrated that a spermatozoa concentration of more than 20 million/ml is essential for reasonable results.
对3418份已知精液特征的冷冻供体精液进行人工授精所获结果进行了分析。与未导致受孕的供体样本相比，来自导致受孕的供体样本在解冻前后的活动精子浓度显著更高。在明确已知导致受孕的精液中发现了更高的浓度。据推测，解冻后的活动精子浓度是最重要的参数，通过将材料分成不同部分可以证明，精子浓度超过2000万/毫升对于获得合理结果至关重要。
