健康小鼠和艾氏腹水瘤荷瘤小鼠尿液中7,8-二氢-6-羟基鲁米嗪的测定

Measurement of urinary 7,8-dihydro-6-hydroxylumazine in healthy and in Ehrlich ascites tumour-bearing mice.

作者信息

Lutz H, Fuchs D, Fuith L, Hausen A, Reibnegger G, Wachter H

出版信息

Hoppe Seylers Z Physiol Chem. 1984 Aug;365(8):895-900. doi: 10.1515/bchm2.1984.365.2.895.

DOI:10.1515/bchm2.1984.365.2.895

Abstract

An estimation of 7,8-dihydro-6-hydroxylumazine in urinary samples of mice by high performance liquid chromatography on reversed phase material is presented. Urinary samples are diluted with water and oxidized by iodine. Chromatographic elution is done isocratically using aqueous buffer containing methanol. Effluent 6-hydroxylumazine is monitored by its fluorescence (382 nm excitation, 449 nm emission). The described method can be performed rapidly with satisfactory accuracy and precision. The electrochemical detection of 7,8-dihydro-6-hydroxylumazine was studied additionally. Total values of daily excreted 7,8-dihydro-6-hydroxylumazine of healthy and of Ehrlich ascites tumour-bearing mice were measured.

摘要

本文介绍了一种采用反相材料通过高效液相色谱法测定小鼠尿液样本中7,8-二氢-6-羟基鲁马嗪的方法。尿液样本用水稀释并用碘氧化。使用含有甲醇的水性缓冲液等度进行色谱洗脱。流出的6-羟基鲁马嗪通过其荧光（382nm激发，449nm发射）进行监测。所描述的方法能够快速进行，具有令人满意的准确度和精密度。此外，还研究了7,8-二氢-6-羟基鲁马嗪的电化学检测。测定了健康小鼠和艾氏腹水瘤荷瘤小鼠每日排泄的7,8-二氢-6-羟基鲁马嗪的总量。