Dwenger A, Friedel R
Fortschr Med. 1978 May 11;96(18):989-96.
Because of the increased number of samples to be processed in digoxin radioimmunoassay for clinical diagnosis and pharmacokinetic studies these analyses require mechanization to a certain extent. A newly developed modular analyzer system is described which is capable of mechanizing radioimmunoassays for digoxin, for example, independent on the kind of reagent kits as well as the separation technique. In dependence on the number of replicates from 60 to 240 samples per hour can be processed. The reaction vessels are placed in belts during the whole assay including centrifugation. The final separation of the liquid and the solid phase after centrifugation can be performed in two different ways: 1. If a conventional automatic gamma counter is used an aliquot of the supernatant is automatically dispensed into the counting vials which are to be closed manually and transferred into the counting device. 2. Further reduction of manual working steps can be achieved by employment of a newly developed discontinuous flow-through gamma counter which is part of the modular analyzer system. An aliquot of the supernatants is automatically flushed into a measuring cuvette installed into a conventional crystal detector. After measurement of the radioactivity and data output the cuvette is rinsed thoroughly. Thereafter the following sample is delivered.
由于用于临床诊断和药代动力学研究的地高辛放射免疫分析中待处理的样本数量增加，这些分析在一定程度上需要机械化。本文描述了一种新开发的模块化分析仪系统，该系统能够使地高辛放射免疫分析机械化，例如，独立于试剂盒的种类以及分离技术。根据重复次数，每小时可处理60至240个样本。在整个分析过程包括离心过程中，反应容器都放置在传送带上。离心后液相和固相的最终分离可以通过两种不同的方式进行：1. 如果使用传统的自动伽马计数器，上清液的一份子会自动分配到计数瓶中，计数瓶需要手动封闭并转移到计数装置中。2. 通过使用新开发的间断流动伽马计数器（它是模块化分析仪系统的一部分），可以进一步减少手动操作步骤。上清液的一份子会自动冲洗到安装在传统晶体探测器中的测量比色皿中。在测量放射性并输出数据后，比色皿会被彻底冲洗。此后，下一个样本被输送进来。
