Lerman S, Megaw J, Gardner K, Takei Y, Franks Y, Gammon A
Invest Ophthalmol Vis Sci. 1984 Nov;25(11):1267-74.
Young (less than 1 year) and old (greater than 15 years) Rhesus monkeys were utilized in this study in order to determine whether ultraviolet (UV) radiation at ambient levels induces psoralen photobinding in primate eyes (in particular the lens and retina). Unilateral aphakia or pseudophakia was induced surgically and the eyes were allowed to heal. The animals then were given a single intraperitoneal injection of 3H 8-methoxypsoralen (8-MOP) and immediately exposed to BLB lights (of measured radiation intensity at the corneal surface). The animals were killed at varying time periods (2-6 weeks), and the eyes were removed immediately. One-half of each cornea and lens was frozen for subsequent optical spectroscopy and the remaining ocular tissues were fixed for histopathologic studies and autoradiography. These data demonstrate that low level UV radiation (less than 0.4 mW/cm2) can cause 8-MOP photobinding to lens proteins and DNA and to aphakic, pseudophakic, and young phakic primate retinas. The older phakic primate lens serves as a protective UV filter and prevents psoralen photobinding within the retina. These data suggest that older aphakes and pseudophakes may require UV radiation protection to prevent direct as well as photosensitized retinal photodamage.
本研究使用了幼年（小于1岁）和老年（大于15岁）的恒河猴，以确定环境水平的紫外线（UV）辐射是否会诱导灵长类动物眼睛（特别是晶状体和视网膜）中的补骨脂素光结合。通过手术诱导单侧无晶状体或假晶状体形成，并让眼睛愈合。然后给动物腹腔注射一次3H 8-甲氧基补骨脂素（8-MOP），并立即暴露于BLB光下（测量角膜表面的辐射强度）。在不同时间段（2-6周）处死动物，并立即摘除眼睛。将每个角膜和晶状体的一半冷冻用于后续的光学光谱分析，其余眼组织固定用于组织病理学研究和放射自显影。这些数据表明，低水平的紫外线辐射（小于0.4 mW/cm2）可导致8-MOP与晶状体蛋白和DNA以及无晶状体、假晶状体和幼年有晶状体灵长类动物视网膜发生光结合。老年有晶状体灵长类动物的晶状体可作为保护性紫外线滤光器，防止补骨脂素在视网膜内发生光结合。这些数据表明，老年无晶状体和假晶状体患者可能需要紫外线辐射防护，以防止直接以及光致敏性视网膜光损伤。
