Shaw P J, Hills G J
J Cell Sci. 1984 Jun;68:271-84. doi: 10.1242/jcs.68.1.271.
The green alga Chlorogonium elongatum, a member of the Volvocales, possesses a crystalline cell wall composed of hydroxyproline-rich glycoprotein similar to the primary cell wall glycoproteins of higher plants. Electron microscopy and computer image processing have been used to determine the crystal structure of the Chlorogonium cell wall in three dimensions to a resolution of 2.0 nm. The structure is composed of heterologous dimers. Each subunit of the dimer comprises a long, thin spacer domain and a large globular domain, which is the site of the intra- and inter-dimer interactions. There are also sites of intersubunit interactions at the opposite ends of the rod domains. We suggest that the rods are composed predominantly of glycosylated polyproline helix, as has been suggested for higher plant cell wall glycoproteins and has been shown for the cell wall glycoprotein of Chlamydomonas reinhardtii, which is closely related to Chlorogonium.
绿藻长形绿梭藻是团藻目的成员，其具有由富含羟脯氨酸的糖蛋白组成的结晶细胞壁，类似于高等植物的初生细胞壁糖蛋白。电子显微镜和计算机图像处理已被用于三维确定长形绿梭藻细胞壁的晶体结构，分辨率为2.0纳米。该结构由异源二聚体组成。二聚体的每个亚基包括一个长而细的间隔结构域和一个大的球状结构域，球状结构域是二聚体内和二聚体间相互作用的位点。在杆状结构域的相对末端也存在亚基间相互作用的位点。我们认为这些杆主要由糖基化的多聚脯氨酸螺旋组成，正如对高等植物细胞壁糖蛋白所提出的那样，并且对于与长形绿梭藻密切相关的莱茵衣藻的细胞壁糖蛋白已得到证实。
