Mair A E, Miller J H
J Clin Hosp Pharm. 1984 Sep;9(3):217-24. doi: 10.1111/j.1365-2710.1984.tb01079.x.
A high-performance liquid chromatographic assay is described for the simultaneous assay of pilocarpine and physostigmine in the presence of their decomposition products. This assay method was used to quantitatively determine these medicaments in an eye drop formulation which had been sterilized by autoclaving at 115 degrees C for 30 min and at 121 degrees C for 15 min. There was no significant loss of pilocarpine whereas physostigmine was degraded. The major decomposition product is shown to be eseroline.
描述了一种高效液相色谱法，用于在毛果芸香碱和毒扁豆碱及其分解产物存在的情况下同时测定它们。该测定方法用于定量测定一种滴眼剂中的这些药物，该滴眼剂已在115℃下高压灭菌30分钟和在121℃下高压灭菌15分钟。毛果芸香碱没有明显损失，而毒扁豆碱发生了降解。主要分解产物显示为毒扁豆灵。
