Holder N, Weekes C
J Embryol Exp Morphol. 1984 Aug;82:217-39.
The regeneration of surgically created mixed-handed limb stumps is examined in the axolotl. Operations were performed in the lower arm and upper arm regions and grafts were allowed to heal for approximately one month prior to amputation or were amputated immediately. In the lower arm group both anterior and posterior limb halves were inverted, whereas only posterior halves were inverted in the upper arm group. Almost all the limbs regenerated were normal in the anterior-posterior axis, whereas a range of limb types were found when the dorsal-ventral axis was analysed using the metacarpal muscle pattern and epidermal Leydig cell number as positional markers. The carpal and forearm muscle patterns were also analysed in order to assess whether the pattern determined from analysis at the metacarpal level reflected that seen at more proximal levels. The results are discussed in terms of the possible role of cell contribution from the stump to the blastema and the relevance of the study to models of pattern regulation.
在墨西哥钝口螈中研究了手术创建的混合手肢体残端的再生情况。手术在小臂和上臂区域进行，移植后让伤口愈合约一个月再进行截肢，或者立即截肢。在小臂组中，前后两半肢体都进行了翻转，而在上臂组中只有后半部分进行了翻转。几乎所有再生的肢体在前后轴上都是正常的，然而，当使用掌骨肌肉模式和表皮莱迪希细胞数量作为位置标记来分析背腹轴时，发现了一系列不同类型的肢体。还分析了腕骨和前臂肌肉模式，以评估从掌骨水平分析确定的模式是否反映了在更近端水平看到的模式。根据残端细胞对芽基的贡献的可能作用以及该研究与模式调节模型的相关性对结果进行了讨论。
