Korneluk R G, Liversage R A
J Embryol Exp Morphol. 1984 Aug;82:9-24.
Regeneration of boneless amputated forearms of adult newts was found to progress at a rate and to a degree comparable to amputated control limbs in which stump bones were not removed. In contrast, regeneration of boneless amputated Xenopus froglet forearms was significantly delayed and did not occur until two to three weeks following amputation. This is in comparison with the initiation of distal cartilage formation observed one week postamputation in control forelimbs of Xenopus froglets. The regeneration of cartilage in boneless forearms of adult newts was found to occur distal to the amputation level. In contrast, distal as well as proximal (centripetal) regeneration of cartilage was observed in the amputated boneless forearms of Xenopus. In froglets and newts, unamputated forelimbs in which forearm bones were extirpated did not initiate cartilage regeneration. Our findings support the hypothesis that forelimb regeneration in Xenopus froglets is primarily a tissue response. In comparison, limb regeneration in the adult newt is predominantly an epimorphic response.
研究发现，成年蝾螈无骨截肢前臂的再生速度和程度与未切除残端骨骼的对照肢体相当。相比之下，非洲爪蟾幼体无骨截肢前臂的再生明显延迟，直到截肢后两到三周才开始。这与在非洲爪蟾幼体对照前肢截肢一周后观察到的远端软骨形成的起始情况形成对比。成年蝾螈无骨前臂的软骨再生发生在截肢水平的远端。相比之下，在非洲爪蟾无骨截肢前臂中观察到了软骨的远端以及近端（向心性）再生。在幼体和蝾螈中，切除前臂骨骼的未截肢前肢并未启动软骨再生。我们的研究结果支持了这样一种假设，即非洲爪蟾幼体的前肢再生主要是一种组织反应。相比之下，成年蝾螈的肢体再生主要是一种形态发生反应。
