Wecker L, Trommer B A
J Neurochem. 1984 Dec;43(6):1762-5. doi: 10.1111/j.1471-4159.1984.tb06107.x.
The effects of dietary choline availability on the transport of choline across the blood-brain barrier (BBB) were investigated using the intracarotid injection technique. Maintenance of rats on choline-deficient, basal choline, or choline-supplemented diets for 28-32 days led to respective increases in blood levels of choline and correlative increases in the velocity of transport of choline measured using a buffer injectate. When serum from these rats was included in the injectate and transport determined in control animals, there was a marked inhibition of choline transport that was related to the concentration of choline in the diets. Results suggest that the activity of the choline carrier at the BBB is antagonized by an inhibitory substance in serum whose concentration or activity may be modified by chronic alterations in circulating levels of choline and whose presence may normally regulate the velocity of choline transport.
采用颈内注射技术研究了膳食中胆碱可利用性对胆碱跨血脑屏障（BBB）转运的影响。将大鼠分别维持在胆碱缺乏、基础胆碱或补充胆碱的饮食上28 - 32天，导致血液中胆碱水平相应升高，并且使用缓冲注射液测定的胆碱转运速度也随之相应增加。当将这些大鼠的血清加入注射液中并在对照动物中测定转运时，胆碱转运受到明显抑制，这种抑制与饮食中胆碱的浓度有关。结果表明，血脑屏障处胆碱载体的活性受到血清中一种抑制性物质的拮抗，该抑制性物质的浓度或活性可能会因循环胆碱水平的慢性改变而改变，并且其存在可能通常调节胆碱的转运速度。
