Frost L, Ghosh P
J Orthop Res. 1984;2(3):207-20. doi: 10.1002/jor.1100020302.
Proteoglycans (PGs) isolated from articular cartilage (AC) of mature rabbits subjected to two or more consecutive intraarticular (IA) injections of sterile saline 24 h apart showed an aggregation defect in the presence of excess hyaluronic acid (HA). Although the PG contents of experimental and control cartilages were indistinguishable, a higher proportion of PGs were extractable from the 3 X IA tissues, as assessed by uronic acid analysis. Proteoglycans from experimental and control cartilages when examined by Sepharose CL-2B chromatography showed two subunit populations, the smaller (KAV = 0.70) containing more ketatan sulphate than the larger (KAV = 0.31). Cultures of AC from IA joints released more 35SO4-labelled PGs into the media over 72 h than control tissues and consisted mainly of PG degradation products although 20% could aggregate in the presence of HA. Examination of PG aggregation 2 weeks after 2 X IA or 3 X IA injections showed that the defect initiated was still present; however, cartilage of immature rabbits was not affected by the 2 X IA procedure.
从成年兔关节软骨（AC）中分离出的蛋白聚糖（PGs），在每隔24小时进行两次或更多次连续关节内（IA）无菌盐水注射后，在存在过量透明质酸（HA）的情况下显示出聚集缺陷。尽管实验软骨和对照软骨的PG含量没有差异，但通过糖醛酸分析评估，3次IA组织中可提取的PG比例更高。通过琼脂糖CL-2B色谱法检测，实验软骨和对照软骨的蛋白聚糖显示出两个亚基群体，较小的亚基（KAV = 0.70）比较大的亚基（KAV = 0.31）含有更多的硫酸角质素。与对照组织相比，IA关节的AC培养物在72小时内释放到培养基中的35SO4标记的PG更多，并且主要由PG降解产物组成，尽管20%在HA存在下可以聚集。在2次IA或3次IA注射2周后检查PG聚集情况，发现最初出现的缺陷仍然存在；然而，未成熟兔的软骨不受2次IA操作的影响。
