Bracken W M, Sharma R P, Bourcier D R
J Toxicol Environ Health. 1984;13(4-6):865-77. doi: 10.1080/15287398409530547.
A bovine kidney cell culture system was used to assess what relationship mercuric chloride (HgCl2) uptake and subcellular distribution had to cytotoxicity. Twenty-four-hour incubations with 0.05-50 micro M HgCl2 elicited a concentration-related, with 1.0 nmol/10(6) cells at the IC50. Measurement of Hg uptake over the 24-h exposure period revealed a multiphasic process. Peak accumulation was attained by 1 h and was followed by extrusion and plateauing of intracellular Hg levels. Least-squares regression analysis of the cytotoxicity and cellular uptake data indicated a potential relationship between the Hg uptake and cytotoxicity (r = 0.91). However, the subcellular distribution of Hg was not concentration-related. Mitochondria and soluble protein fractions accounted for greater than 65% of the cell-associated Hg at all concentrations. The remaining Hg was distributed between the microsomal (6-10%) and nuclear and cell debris (11-22%) fractions at all concentrations tested. Less than 20% of the total cell-associated Hg was bound with metallothionein-like protein.
使用牛肾细胞培养系统来评估氯化汞（HgCl2）的摄取及亚细胞分布与细胞毒性之间的关系。用0.05 - 50 μM HgCl2孵育24小时会引发浓度相关性反应，半数抑制浓度（IC50）时为1.0 nmol/10(6) 个细胞。在24小时暴露期内对汞摄取的测量显示这是一个多相过程。1小时时达到峰值积累，随后细胞内汞水平出现排出和平稳状态。对细胞毒性和细胞摄取数据进行的最小二乘法回归分析表明汞摄取与细胞毒性之间存在潜在关系（r = 0.91）。然而，汞的亚细胞分布与浓度无关。在所有浓度下，线粒体和可溶性蛋白组分占细胞相关汞的比例均超过65%。在所有测试浓度下，其余的汞分布在微粒体（6 - 10%）以及细胞核和细胞碎片（11 - 22%）组分之间。与细胞相关的总汞中，与金属硫蛋白样蛋白结合的不到20%。
