台湾眼镜蛇（中华眼镜蛇）毒液中心脏毒素二硫键的位置

Location of disulfide bridges in cardiotoxin from the venom of Formosan cobra (NAJA NAJA ATRA).

作者信息

Narita K, Cheng K L, Chang W C, Lo T B

出版信息

Int J Pept Protein Res. 1978 Mar;11(3):229-37. doi: 10.1111/j.1399-3011.1978.tb02843.x.

DOI:10.1111/j.1399-3011.1978.tb02843.x

Abstract

The location of the four disulfide bridges in cardiotoxin from the venom of the Formosan cobra, Naja naja atra, was determined by isolating cystine-containing peptides from the enzymic and partial acid hydrolyzates of the intact toxin. The results indicate that the disulfide bridges are formed by half cystine residues 3--21, 14--38, 42--53, and 54--59. The amino acid sequence at the position 48--49 was revised to -Leu-Val- instead of Val-Leu- as reported previously.

摘要

通过从完整毒素的酶解产物和部分酸解产物中分离含胱氨酸的肽段，确定了台湾眼镜蛇（Naja naja atra）毒液中心脏毒素内4个二硫键的位置。结果表明，二硫键由半胱氨酸残基3 - 21、14 - 38、42 - 53和54 - 59形成。48 - 49位的氨基酸序列由先前报道的-Val-Leu-修正为-Leu-Val-。