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[通过放射免疫沉淀法检测血清中抗黄蜂毒液IgG。在脱敏治疗期间应用于患者]

[Serum assay of anti-wasp venom IgG by radioimmunoprecipitation. Application to patients during desensitization].

作者信息

Guilloux L, Ville G

出版信息

Pathol Biol (Paris). 1984 Sep;32(7):785-90.

PMID:6493814
Abstract

Rapid, specific and reproducible radioimmunoprecipitation assay (RIP) for measuring IgG antibodies against Yellow Jacket Venom (YJV) has been developed. The YJV-I125 is added to serum sample and the immune complexes are precipitated by a second antibody with polyethylene glycol. IgG antibodies were measured by RIP in 24 patients receiving Yellow Jacket Venom immunotherapy. RIP assays were compared for 159 sera to a solid phase procedure (RAST IgG).

摘要

已开发出一种快速、特异且可重复的放射免疫沉淀测定法(RIP),用于检测抗黄夹克毒液(YJV)的IgG抗体。将YJV-I125加入血清样本中,免疫复合物用聚乙二醇和第二抗体沉淀。采用RIP法检测了24例接受黄夹克毒液免疫疗法患者的IgG抗体。将159份血清的RIP测定结果与一种固相检测方法(RAST IgG)进行了比较。

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