Gromysz H, Karczewski W A
Respir Physiol. 1984 Aug;57(2):225-33. doi: 10.1016/0034-5687(84)90095-1.
In 22 cats anaesthetized with halothane, vagotomised, paralyzed with d-tubocurarine and artificially ventilated, the medulla was split in the midline and the response of phrenic motoneurones, efferent vagus nerve and medullary respiratory neurones to hypercapnia was studied. In 18 successful experiments the mid-sagittal incision abolished all electrical activities but an inhalation of a 5% CO2-oxygen mixture promptly restored rhythmic firing in both the medullary units and efferent nerves when PaCO2 reached 55 mm Hg on the average (mean pH = 7.20). Switching the ventilation back to normocapnia was followed by a gradual disappearance of the activities usually when PaCO2 and pH returned to control values. We conclude that splitting the brainstem in cat affects the respiratory rhythmogenesis, presumably by increasing the CO2-threshold of medullary respiratory neurones.
在22只使用氟烷麻醉、切断迷走神经、用右旋筒箭毒碱使其麻痹并进行人工通气的猫身上，将延髓在中线处切开，研究膈运动神经元、迷走神经传出纤维和延髓呼吸神经元对高碳酸血症的反应。在18次成功的实验中，正中矢状切口消除了所有电活动，但当平均动脉血二氧化碳分压（PaCO2）达到55 mmHg（平均pH = 7.20）时，吸入5%二氧化碳-氧气混合气体能迅速恢复延髓单位和传出神经的节律性放电。通常当PaCO2和pH恢复到对照值时，将通气恢复到正常碳酸水平后，活动会逐渐消失。我们得出结论，在猫身上切开脑干会影响呼吸节律的产生，可能是通过提高延髓呼吸神经元的二氧化碳阈值来实现的。
