Teokharova M P, Andonov A P, Mineva E G, Draganov P I
Vopr Virusol. 1984 Jul-Aug;29(4):483-7.
Two experimental lots of hepatitis B vaccine were prepared by purification of HBsAg from human plasma, inactivation at 100 degrees C for 2 min and at 37 degrees C for 72 hours with formalin in a concentration of 1:4000. The former lot comprises purified HBsAg (40 mg/ml) adsorbed with 0.32 mg % Al(OH)3, the latter consists of purified HBsAg stabilized with 0.1% human albumin and adsorbed with 1 mg% Al(OH)3. The immune response after vaccination with the first lot was observed in 54.54% of the vaccinees. The second vaccine after 3 administrations of 80 micrograms/ml each produced a very good primary and a very good secondary immune response. Both vaccines are nonreactogenic and well tolerated by the vaccinees.
通过从人血浆中纯化乙肝表面抗原（HBsAg），在100℃下灭活2分钟，然后在37℃下用浓度为1:4000的福尔马林灭活72小时，制备了两批实验性乙肝疫苗。第一批疫苗包含吸附有0.32mg%氢氧化铝（Al(OH)3）的纯化HBsAg（40mg/ml），第二批疫苗由用0.1%人白蛋白稳定并吸附有1mg% Al(OH)3的纯化HBsAg组成。接种第一批疫苗后，54.54%的接种者出现了免疫反应。第二批疫苗在每次接种80微克/毫升、共接种3次后，产生了非常良好的初次免疫反应和二次免疫反应。两种疫苗均无反应原性，接种者耐受性良好。
