电鳗乙酰胆碱酯酶。低离子强度聚集体的生化及电子显微镜表征
Electrophorus acetylcholinesterase. Biochemical and electron microscope characterization of low ionic strength aggregates.
作者信息
Cartaud J, Bon S, Massoulié J
出版信息
J Cell Biol. 1978 May;77(2):315-22. doi: 10.1083/jcb.77.2.315.
Abstract
The "tailed" molecules of Electrophorus (electric eel) acetylcholinesterase aggregate under conditions of low ionic strength. These aggregates have been studied by sedimentation analysis and high-resolution electron microscopy. They consist of bundles of at least half a dozen molecules, the tails of which are packed side by side, to form the core of the structure. Although aggregation is normally fully reversible, aggregates were irreversibly stabilized by methylene blue-sensitized photo-oxidation. This process was shown to consist of a singlet oxygen oxidation reaction and probably involves methionine or histidine residues. It did not modify the structural or hydrodynamic characteristics of the aggregates.
摘要
电鳗乙酰胆碱酯酶的“带尾”分子在低离子强度条件下会聚集。这些聚集体已通过沉降分析和高分辨率电子显微镜进行了研究。它们由至少半打分子的束组成，这些分子的尾部并排堆积，形成结构的核心。尽管聚集通常是完全可逆的，但聚集体通过亚甲蓝敏化的光氧化被不可逆地稳定下来。该过程被证明由单线态氧氧化反应组成，可能涉及甲硫氨酸或组氨酸残基。它没有改变聚集体的结构或流体动力学特征。
相似文献
1
Electrophorus acetylcholinesterase. Biochemical and electron microscope characterization of low ionic strength aggregates.电鳗乙酰胆碱酯酶。低离子强度聚集体的生化及电子显微镜表征
J Cell Biol. 1978 May;77(2):315-22. doi: 10.1083/jcb.77.2.315.
2
The dependence of acetylcholinesterase aggregation at low ionic strength upon a polyanionic component.低离子强度下乙酰胆碱酯酶聚集对多阴离子成分的依赖性。
Eur J Biochem. 1978 Apr;85(1):1-14. doi: 10.1111/j.1432-1033.1978.tb12207.x.
3
[Electron microscopic studies on stretched and globular acetylcholinesterase molecules of the electric eel (Electrophorus electricus)].[电鳗（电鳐）拉伸和球状乙酰胆碱酯酶分子的电子显微镜研究]
Eur J Biochem. 1973 May 2;34(3):539-47. doi: 10.1111/j.1432-1033.1973.tb02792.x.
4
Attempted direct visualization of negatively stained amplified immune complex of synaptic acetylcholinesterase using cryoultramicrotomy sections.尝试使用冷冻超薄切片术对经负染的突触乙酰胆碱酯酶扩增免疫复合物进行直接可视化观察。
Histochemistry. 1981;72(3):369-75. doi: 10.1007/BF00501779.
5
Molecular structures of acetylcholinesterase from electric organ tissue of the electric eel.电鳗电器官组织中乙酰胆碱酯酶的分子结构。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2473-6. doi: 10.1073/pnas.70.9.2473.
6
Electric eel acetylcholinesterase: a multisubunit enzyme containing a collagen tail.电鳗乙酰胆碱酯酶：一种含有胶原尾巴的多亚基酶。
Monogr Neural Sci. 1980;7:55-69. doi: 10.1159/000388814.
7
Observations on the innervated face of the electrocyte of the main organ of the electric eel (Electrophorus electricus L.).关于电鳗（Electrophorus electricus L.）主要发电器官电细胞受神经支配面的观察。
Cell Tissue Res. 1980;213(1):69-80. doi: 10.1007/BF00236921.
8
Collagenase sensitivity and aggregation properties of Electrophorus acetylcholinesterase.
Eur J Biochem. 1978 Aug 15;89(1):89-94. doi: 10.1111/j.1432-1033.1978.tb20899.x.
9
Dumbbell-shaped associations of tailed Electrophorus acetylcholinesterase molecules.
Mol Biol Rep. 1978 Feb 28;4(1):61-3. doi: 10.1007/BF00775183.
10
Ionic strength dependence of the inhibition of acetylcholinesterase activity by Al3+.
Biophys Chem. 1985 Mar;21(3-4):261-4. doi: 10.1016/0301-4622(85)80013-2.
引用本文的文献
1
The concept of allosteric interaction and its consequences for the chemistry of the brain.变构相互作用的概念及其对大脑化学的影响。
J Biol Chem. 2013 Sep 20;288(38):26969-26986. doi: 10.1074/jbc.X113.503375. Epub 2013 Jul 22.
2
Consequences of alkaline treatment for the ultrastructure of the acetylcholine-receptor-rich membranes from Torpedo marmorata electric organ.碱性处理对电鳐电器官富含乙酰胆碱受体的膜超微结构的影响。
J Cell Biol. 1981 Aug;90(2):418-26. doi: 10.1083/jcb.90.2.418.
3
Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.电鳐突触后膜快速冷冻、深度蚀刻和旋转复型后乙酰胆碱受体的组织
J Cell Biol. 1979 Jul;82(1):150-73. doi: 10.1083/jcb.82.1.150.
4
Asymmetric and globular forms of acetylcholinesterase in mammals and birds.哺乳动物和鸟类中乙酰胆碱酯酶的不对称和球状形式。
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2546-50. doi: 10.1073/pnas.76.6.2546.
本文引用的文献
1
A new and rapid colorimetric determination of acetylcholinesterase activity.一种新的快速比色法测定乙酰胆碱酯酶活性。
Biochem Pharmacol. 1961 Jul;7:88-95. doi: 10.1016/0006-2952(61)90145-9.
2
Unambiguous evidence for the participation of singlet oxygen ( 1 ) in photodynamic oxidation of amino acids.单线态氧（1）参与氨基酸光动力氧化的确凿证据。
Photochem Photobiol. 1972 Aug;16(2):117-24. doi: 10.1111/j.1751-1097.1972.tb07343.x.
3
[Acetylcholinesterase of fish electric organs (torpedo and electric eel); membrane complexes].[鱼类电器官（电鳐和电鳗）的乙酰胆碱酯酶；膜复合物]
Eur J Biochem. 1969 Dec;11(3):441-55. doi: 10.1111/j.1432-1033.1969.tb00794.x.
4
Phospholipids in "native" Electrophorus acetylcholinesterase.
FEBS Lett. 1973 Oct 1;36(1):12-6. doi: 10.1016/0014-5793(73)80326-6.
5
[Properties of elongated acetylcholinesterase molecules in solution. Stokes radius, density, and mass].[溶液中细长型乙酰胆碱酯酶分子的性质。斯托克斯半径、密度和质量]
Eur J Biochem. 1973 Jun;35(2):372-9. doi: 10.1111/j.1432-1033.1973.tb02849.x.
6
[Electron microscopic studies on stretched and globular acetylcholinesterase molecules of the electric eel (Electrophorus electricus)].[电鳗（电鳐）拉伸和球状乙酰胆碱酯酶分子的电子显微镜研究]
Eur J Biochem. 1973 May 2;34(3):539-47. doi: 10.1111/j.1432-1033.1973.tb02792.x.
7
Molecular structures of acetylcholinesterase from electric organ tissue of the electric eel.电鳗电器官组织中乙酰胆碱酯酶的分子结构。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2473-6. doi: 10.1073/pnas.70.9.2473.
8
Biological significance of the intermolecular crosslinks of collagen.胶原蛋白分子间交联的生物学意义。
Nature. 1974 Sep 13;251(5471):105-9. doi: 10.1038/251105a0.
9
Use of uranyl formate as a negative stain.使用甲酸双氧铀作为负染剂。
J Mol Biol. 1965 Sep;13(2):606. doi: 10.1016/s0022-2836(65)80124-3.
10
Fine structure of electric ell acetylcholinesterase.电鳗乙酰胆碱酯酶的精细结构。
Brain Res. 1975 Apr 25;88(1):127-30. doi: 10.1016/0006-8993(75)90959-2.
Zotero 插件