Cartaud J, Bon S, Massoulié J
J Cell Biol. 1978 May;77(2):315-22. doi: 10.1083/jcb.77.2.315.
The "tailed" molecules of Electrophorus (electric eel) acetylcholinesterase aggregate under conditions of low ionic strength. These aggregates have been studied by sedimentation analysis and high-resolution electron microscopy. They consist of bundles of at least half a dozen molecules, the tails of which are packed side by side, to form the core of the structure. Although aggregation is normally fully reversible, aggregates were irreversibly stabilized by methylene blue-sensitized photo-oxidation. This process was shown to consist of a singlet oxygen oxidation reaction and probably involves methionine or histidine residues. It did not modify the structural or hydrodynamic characteristics of the aggregates.
电鳗乙酰胆碱酯酶的“带尾”分子在低离子强度条件下会聚集。这些聚集体已通过沉降分析和高分辨率电子显微镜进行了研究。它们由至少半打分子的束组成，这些分子的尾部并排堆积，形成结构的核心。尽管聚集通常是完全可逆的，但聚集体通过亚甲蓝敏化的光氧化被不可逆地稳定下来。该过程被证明由单线态氧氧化反应组成，可能涉及甲硫氨酸或组氨酸残基。它没有改变聚集体的结构或流体动力学特征。
