腺嘌呤核苷酸对大鼠睾丸粗线期初级精母细胞中甘油醛-3-磷酸脱氢酶的抑制作用。
Inhibition of glyceraldehyde 3-phosphate dehydrogenase by adenine nucleotides in pachytene primary spermatocytes from rat testes.
作者信息
Nakamura M, Okinaga S, Arai K
出版信息
Andrologia. 1984 Sep-Oct;16(5):446-50. doi: 10.1111/j.1439-0272.1984.tb00395.x.
The effect of adenine nucleotides on the catalytic activity of spermatocyte glyceraldehyde 3-phosphate dehydrogenase (GA3PDH) was studied for its significance to metabolic regulation. In the presence of glucose (10 mM), the ATP level declined whereas the ADP and AMP levels remained unchanged. During incubation with glucose, fructose 1,6-bisphosphate (fruc 1,6-P2) and dihydroxyacetone phosphate (DHAP) were accumulated markedly. GA3PDH was inhibited by ATP (Ki = 2.27 mM), ADP (Ki = 1.21 mM) and AMP (Ki = 0.73 mM) competitively with NAD (Km = 0.24 mM). The results suggest that glycolysis in spermatocytes is regulated by GA3PDH.
研究了腺嘌呤核苷酸对精母细胞3-磷酸甘油醛脱氢酶（GA3PDH）催化活性的影响，以探讨其对代谢调节的意义。在存在葡萄糖（10 mM）的情况下，ATP水平下降，而ADP和AMP水平保持不变。在与葡萄糖孵育期间，1,6-二磷酸果糖（fruc 1,6-P2）和磷酸二羟丙酮（DHAP）显著积累。GA3PDH被ATP（Ki = 2.27 mM）、ADP（Ki = 1.21 mM）和AMP（Ki = 0.73 mM）与NAD（Km = 0.24 mM）竞争性抑制。结果表明，精母细胞中的糖酵解受GA3PDH调节。
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