Burgess C
J Chromatogr. 1978 Feb 11;149:233-40. doi: 10.1016/s0021-9673(00)80989-9.
The optimisation of conditions for the application of reversed-phase high-performance liquid chromatography (HPLC) to the analysis of steroid products is discussed. The influence of temperature and flow-rate on column efficiency are described. At 60 degrees, the reduced velocity required to give maximum efficiency, is approximately twice that found at room temperature, allowing faster through-put. (hv) curves are reported for 5-micron Spherisorb-ODS and Hypersil-ODS material for corticosteroids at 60 degrees. On-column injection techniques are discussed and data presented, showing that for corticosteroids on-column loop injection can be efficient as septum injection using the techniques described.
讨论了反相高效液相色谱法（HPLC）应用于甾体产品分析的条件优化。描述了温度和流速对柱效的影响。在60摄氏度时，达到最大柱效所需的折合流速约为室温下的两倍，从而可实现更快的通量。报告了5微米Spherisorb - ODS和Hypersil - ODS材料在60摄氏度下用于皮质类固醇的（hv）曲线。讨论了柱上进样技术并给出了数据，结果表明，对于皮质类固醇，使用所述技术进行柱上定量环进样可与隔垫进样一样有效。
