Koester J, Bussmann R, Barz W
Arch Biochem Biophys. 1984 Nov 1;234(2):513-21. doi: 10.1016/0003-9861(84)90298-4.
A malonyltransferase which catalyzes the malonylation of isoflavone 7-O-glucosides in position 6 of the glucose moiety using malonyl-coenzyme A as acyl donor has been purified 157-fold from 4-day-old roots of chick pea (Cicer arietinum L.). The enzyme showed a pH optimum of 8.0 and a molecular weight of 112,000. The Km for malonylcoenzyme A was 48 microM and, for the chick pea isoflavones biochanin A and formononetin, 36 and 24 microM, respectively. Various other isoflavone, flavone, and flavonol 7-O-glucosides and chalcone 4'-O-glucosides were much poorer substrates. Flavonol 3-O-glucosides and isoflavone 4'-O-glucosides were not malonylated by the malonyltransferase.
已从鹰嘴豆（Cicer arietinum L.）4日龄的根中纯化出一种丙二酰基转移酶，该酶以丙二酰辅酶A作为酰基供体，催化异黄酮7-O-葡萄糖苷葡萄糖部分6位的丙二酰化反应，纯化倍数达157倍。该酶的最适pH为8.0，分子量为112,000。丙二酰辅酶A的Km为48 microM，鹰嘴豆异黄酮染料木黄酮和芒柄花素的Km分别为36 microM和24 microM。其他各种异黄酮、黄酮和黄酮醇7-O-葡萄糖苷以及查耳酮4'-O-葡萄糖苷是较差的底物。黄酮醇3-O-葡萄糖苷和异黄酮4'-O-葡萄糖苷不能被丙二酰基转移酶丙二酰化。
