Matern U, Feser C, Heller W
Arch Biochem Biophys. 1984 Nov 15;235(1):218-27. doi: 10.1016/0003-9861(84)90271-6.
Three distinct N-malonyltransferases were purified from peanut seedlings, accepting either anthranilic acid, D-tryptophan, or 3,4-dichloroaniline, respectively, as a substrate. Partially purified malonyl-CoA:D-tryptophan malonyltransferase also catalyzed the formation of the corresponding malonic acid conjugate when 1-aminocyclopropane-1-carboxylic acid was employed as a substrate. These N-malonyltransferases were clearly distinguished from several O-malonyltransferase activities also present in the same seedlings. N-Malonic acid conjugates have been previously isolated from peanut either as a natural constituent or after feeding with xenobiotics. By analogy to the results reported with cultured parsley cells, multiple malonyltransferases in peanut may have a role in vacuolar transport. Crude extracts of young peanut seedlings were incapable of hydrolyzing the respective N-malonic acid conjugates. However, dialyzed extracts of older plants released malonic acid from malonyl-1-aminocyclopropane-1-carboxylic acid but not from malonyl-3,4-dichloroaniline, suggesting that some N-malonic acid conjugates may be metabolized in plants which are approaching senescence.
从花生幼苗中纯化出了三种不同的N-丙二酰基转移酶，它们分别以邻氨基苯甲酸、D-色氨酸或3,4-二氯苯胺作为底物。部分纯化的丙二酰辅酶A：D-色氨酸丙二酰基转移酶在以1-氨基环丙烷-1-羧酸作为底物时，也催化形成相应的丙二酸共轭物。这些N-丙二酰基转移酶与同一幼苗中也存在的几种O-丙二酰基转移酶活性明显不同。N-丙二酸共轭物先前已从花生中作为天然成分或在喂食异源生物后分离出来。类比在培养的欧芹细胞中报道的结果，花生中的多种丙二酰基转移酶可能在液泡运输中起作用。幼嫩花生幼苗的粗提物无法水解各自的N-丙二酸共轭物。然而，较老植株的透析提取物可从丙二酰-1-氨基环丙烷-1-羧酸中释放出丙二酸，但不能从丙二酰-3,4-二氯苯胺中释放，这表明一些N-丙二酸共轭物可能在接近衰老的植株中被代谢。
