Bianchetti G, Mitchard M, Morselli P L
J Chromatogr. 1978 May 11;152(1):87-95. doi: 10.1016/s0021-9673(00)85337-6.
A procedure is described for the determination of tiflorex and its metabolite nortiflorex in biological specimens. The compounds are converted into their trichoroacetyl derivatives, which are separated on a glass column packed with 3% OV-17 on Gas-Chrom Q, and measured with an electron-capture detector. The mechanism was investigated by gas chromatography-mass spectrometry. The method is rapid, sensitive for concentrations of 1 ng/ml and has been used to measure tiflorex and its metabolite in rat plasma after intravenous administration and in human volunteers after administration by the oral route.
本文描述了一种测定生物样本中替氟雷司及其代谢产物去甲替氟雷司的方法。这些化合物被转化为其三氯乙酰衍生物，在填充有3% OV - 17的Gas - Chrom Q玻璃柱上进行分离，并用电子捕获检测器进行测定。通过气相色谱 - 质谱联用对其机制进行了研究。该方法快速，对浓度为1 ng/ml时敏感，已用于测定大鼠静脉注射给药后血浆中替氟雷司及其代谢产物，以及人类志愿者口服给药后的情况。
