[高压液相色谱法（HPLC）同时测定4种抗惊厥药物]
[Simultaneous determination of 4 anticonvulsant drugs by high pressure liquid chromatography (HPLC)].
作者信息
Andriolo A, Germek O A, Pereira A B
出版信息
Arq Neuropsiquiatr. 1984 Sep;42(3):203-8. doi: 10.1590/s0004-282x1984000300002.
We describe a sensitive and precise method for the simultaneous high pressure liquid-chromatography determination of phenobarbital, diphenylhydantoin, primidone, and carbamazepine in serum. The drugs are extracted into chloroform, dried and dissolved in the mobile phase. The drugs are eluted from a reverse-phase column with methanol/water and detected by their absorption at 214 nm. Concentrations are estimated from their peak heights. The results, when compared with those by enzyme-immunoassay, gave correlation coefficients of 0.990 for phenobarbital, 0.993 for diphenylhydantoin, 0.992 for primidone and 0.994 for carbamazepine.
我们描述了一种灵敏且精确的方法，用于同时采用高压液相色谱法测定血清中的苯巴比妥、苯妥英、扑米酮和卡马西平。药物被萃取至氯仿中，干燥后溶解于流动相。药物通过甲醇/水从反相柱上洗脱，并通过其在214nm处的吸收进行检测。浓度根据其峰高进行估算。与酶免疫测定法的结果相比，苯巴比妥的相关系数为0.990，苯妥英为0.993，扑米酮为0.992，卡马西平为0.994。
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