Pajares M A, Alemany S, Varela I, Marin Cao D, Mato J M
Biochem J. 1984 Oct 1;223(1):61-6. doi: 10.1042/bj2230061.
An enzyme that catalyses the three-step methylation of phosphatidylethanolamine to phosphatidylcholine as well as the methylation of fatty acids and that uses S-adenosylmethionine as the methyl donor has been purified about 200-fold from rat liver. Irradiation of the purified enzyme with a short-wavelength u.v. light in the presence of [methyl-3H]8-azido-S-adenosylmethionine followed by electrophoresis results in the incorporation of radioactivity into a single protein band of about 25 kDa. It is concluded that a single catalytic subunit catalyses the conversion of phosphatidylethanolamine into phosphatidylcholine and fatty acid methylation.
一种催化磷脂酰乙醇胺三步甲基化生成磷脂酰胆碱以及脂肪酸甲基化反应，并以S-腺苷甲硫氨酸作为甲基供体的酶已从大鼠肝脏中纯化出来，纯化倍数约为200倍。在[甲基-³H]8-叠氮基-S-腺苷甲硫氨酸存在的情况下，用短波长紫外线照射纯化后的酶，然后进行电泳，结果放射性掺入到一条约25 kDa的单一蛋白条带中。由此得出结论，单一催化亚基催化磷脂酰乙醇胺向磷脂酰胆碱的转化以及脂肪酸甲基化反应。
