Nielsen P E, Hansen J B, Buchardt O
Biochem J. 1984 Oct 15;223(2):519-26. doi: 10.1042/bj2230519.
A novel cleavable photo-cross-linking reagent, N-(2-methoxy-6-azidoacridin-9-yl)-N'-(4-azidobenzoyl)cystamine, for analysis of protein-nucleic acid interactions, has been synthesized. The reagent contains two photosensitive groups that can be activated sequentially. The azidoacridinyl moiety is sensitive to u.v. and visible light (lambda less than or equal to 450 nm), whereas the azidobenzoyl part needs higher-energy light (lambda less than or equal to 350 nm). Furthermore, the disulphide bridge connecting the two photoactive groups can be cleaved by reduction with mercaptans. The reagent is shown to induce cleavable cross-links between all five major histones and DNA in chromatin from Ehrlich ascites cells on activation with long-wavelength u.v. light (lambda greater than 300 nm) at an efficiency of approximately 3% of the added reagent.
一种用于分析蛋白质 - 核酸相互作用的新型可裂解光交联试剂N-(2-甲氧基-6-叠氮吖啶-9-基)-N'-(4-叠氮苯甲酰基)胱胺已被合成。该试剂含有两个可依次激活的光敏基团。叠氮吖啶部分对紫外光和可见光敏感（波长小于或等于450nm），而叠氮苯甲酰部分需要更高能量的光（波长小于或等于350nm）。此外，连接两个光活性基团的二硫键可以通过硫醇还原裂解。在用长波长紫外光（波长大于300nm）激活后，该试剂能在艾氏腹水细胞染色质中的所有五种主要组蛋白和DNA之间诱导可裂解交联，效率约为所加试剂的3%。
