Leoni S, Mangiantini M T, Marino M, Pulcinelli F, Spagnuolo S
Boll Soc Ital Biol Sper. 1984 Aug 31;60(8):1481-7.
The total protein and glycoprotein in electrophoretic patterns of rat liver endoplasmic reticulum we studied in peculiar physiological conditions as perinatal development, pregnancy and liver rigeneration. Previous work in these experimental systems showed a changed microsome lipid composition and modified activities of some ER membrane bound enzymes. The microsomes obtained from foetal and neonatal liver show a modified electrophoretic pattern of both total protein and glycoprotein of low weight with respect to the adult animal. As to the microsomes of 8h and 12h regenerating liver modifications are observed only in the pattern of glycoprotein in the molecular weight range.
我们研究了大鼠肝脏内质网电泳图谱中的总蛋白和糖蛋白,这些研究是在围产期发育、妊娠和肝脏再生等特殊生理条件下进行的。此前在这些实验系统中的研究表明,微粒体脂质组成发生了变化,一些内质网膜结合酶的活性也有所改变。与成年动物相比,从胎儿和新生肝脏中获得的微粒体显示出低分子量的总蛋白和糖蛋白的电泳图谱有所改变。至于再生8小时和12小时的肝脏微粒体,仅在分子量范围内的糖蛋白图谱中观察到了变化。