Muir M, Chanarin I
Br J Haematol. 1984 Nov;58(3):517-23. doi: 10.1111/j.1365-2141.1984.tb03999.x.
Rats were exposed to a mixture of oxygen and nitrous oxide (1/1) for up to 18 d. There was a marked fall in both the serum and liver cobalamin levels. There was a significant rise in the concentration of microbiologically-inactive cobalamins in serum 24 h after exposure to N2O. The proportion of microbiologically-inactive cobalamin in liver increased throughout the period of exposure. We were unable to show a similar effect in sera from patients inhaling N2O for 24 h, nor by exposing rat plasma to N2O for 24 h. It was concluded that microbiologically-inactive cobalamins are produced in vivo from endogenous cobalamins and that one form appears to be a product of cobalamin oxidation.
将大鼠暴露于氧气和一氧化二氮的混合气体（1/1）中长达18天。血清和肝脏中的钴胺素水平均显著下降。暴露于一氧化二氮24小时后，血清中微生物学上无活性的钴胺素浓度显著升高。在整个暴露期间，肝脏中微生物学上无活性的钴胺素比例增加。我们未能在吸入一氧化二氮24小时的患者血清中显示出类似的效果，也未能通过将大鼠血浆暴露于一氧化二氮24小时来显示出类似效果。得出的结论是，微生物学上无活性的钴胺素是由内源性钴胺素在体内产生的，并且其中一种形式似乎是钴胺素氧化的产物。
