来自艾氏腹水瘤细胞的经微球菌核酸酶处理的无细胞提取物中的翻译。起始因子eIF-2B的刺激作用。
Translation in micrococcal nuclease-treated cell-free extracts from Ehrlich ascites tumor cells. Stimulation by initiation factor eIF-2B.
作者信息
Person A, Nielsen P, Beaud G, Trachsel H
出版信息
Biochim Biophys Acta. 1984 Nov 22;783(2):152-7. doi: 10.1016/0167-4781(84)90007-1.
PMID:6498200
Abstract
Translation of exogenous mRNAs in micrococcal nuclease-treated extracts from Ehrlich ascites tumor cells is greatly stimulated by the addition of crude initiation factors or initiation factors eIF-2B and eIF-2 containing eIF-2B. The requirement for exogenous eIF-2B in micrococcal nuclease-treated extracts does not result from either loss or enhanced phosphorylation of eIF-2 during incubation.
摘要
在经微球菌核酸酶处理的艾氏腹水瘤细胞提取物中，添加粗制起始因子或包含eIF-2B的起始因子eIF-2B和eIF-2可极大地刺激外源mRNA的翻译。在经微球菌核酸酶处理的提取物中对外源eIF-2B的需求并非源于孵育过程中eIF-2的丢失或磷酸化增强。
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