Kaneko K, Fujimori S, Kamatani N, Akaoka I
Biochim Biophys Acta. 1984 Nov 28;802(2):169-74. doi: 10.1016/0304-4165(84)90158-2.
In an attempt to study, in human body, the metabolism of 5'-methylthioadenosine, a byproduct of the polyamine biosynthesis, we examined whether this nucleoside is excreted into urine, and, if so, whether the amount increases when the synthesis of polyamines increases. We found that 5'-methylthioadenosine is a natural nucleoside in human urine as evidenced by two separate chromatography systems as well as the analysis of the acetylated compound by gas chromatography-mass spectrometry. Preliminary study did not show the elevation of urinary 5'-methylthioadenosine level in malignant patients, suggesting that the cleaving enzyme, 5'-methylthioadenosine phosphorylase, very efficiently removes this nucleoside in vivo.
为了在人体中研究多胺生物合成的副产物5'-甲硫基腺苷的代谢，我们检测了该核苷是否会排泄到尿液中，以及如果是这样，当多胺合成增加时其含量是否会增加。我们发现，通过两种独立的色谱系统以及气相色谱-质谱联用对乙酰化化合物的分析证明，5'-甲硫基腺苷是人类尿液中的一种天然核苷。初步研究未显示恶性肿瘤患者尿液中5'-甲硫基腺苷水平升高，这表明裂解酶5'-甲硫基腺苷磷酸化酶在体内能非常有效地清除这种核苷。
